BiochemLabF2012.SecondExamReviewQuestions

Ponceaustain bprimaryantibody csecondaryantibody

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: ing
the
cells
we
utilized
for
Western
blotting.

Also,
describe
what
happens,
with
 respect
to
the
expression
of
the
fusion
gene,
when
the
media
does
not
contain
IPTG.
 
 
 4.
Describe
the
functions
of
each
of
the
following
in
our
Western
blotting
experiment:
 
 A.
Ponceau
stain
 B.
Primary
antibody
 C.
Secondary
antibody
 D.
Blocking
buffer
 E.
Horseradish
peroxidase
 F.
4‐chloro‐1‐naphthol
 
 
 5.
Describe
why
proteins
move
through
the
gel
in
SDS/P...
View Full Document

Ask a homework question - tutors are online