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TLC Power Point - Organic Chemistry I Lab Lab#5 Thin Layer...

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Organic Chemistry I - Lab Organic Chemistry I - Lab Lab #5 - Thin Layer Chromatography of Analgesics This is the first of three labs this semester which will employ chromatographic methods for the separation & identification of multiple components in a complex mixture.
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Chromatography Chromatography “Color Writing” “Color Writing” general, versatile method for separation of components in a mixture identification of "unknowns" by comparison with reference samples no restrictions on sample type - organic, inorganic, biological, or medical high sensitivity - detection of μ g amounts or less (10 -6 g) Types of Chromatographic Methods Essential Components 1. TLC - Thin Layer Chromatography 1. stationary phase 2. LC - Liquid Chromatography 2. mobile phase HPLC - High Performance Liquid Chrom. 3. sample mixture 3. GC - Gas Chromatography
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Chromatography Chromatography Stationary Phase Mobile Phase What does it do? stays put! flows around SP What is it? fine solid with fluid (liquid or gas) (physical form)
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