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PUSAT PENGAJIAN SAINS KAJIHAYAT(School of Biological Sciences)UNIVERSITI SAINS MALAYSIABOI207/3 – GENERAL MICROBIOLOGYExperiment 4 : Pure Culture TechniqueName: NUR ADI HAIKAL BIN ZUBER (Group 9)[email protected]Group members1.NORZARIFAH BINTI AHMAD (129961)2.NUR ADNIN BINTI MAT RIPA (129963)3.NUR ALIAH BINTI MOHAMAD JOHARI(131260)4.NUR AQILA BINTI MAZUPI (129964)Matric Number: 129962NRIC: 960711-08-6305Lecturer (lecture): Dr Nethia Mohana KumaranProfessor Dr Mohd Nazalan NajimuddinDr Masratul HawaDate submitted:sample1.0 INTRODUCTION1SAMPLE
It is possible to culture microbes if a suitable culture media is present. A culture medium isa solid or liquid preparation used to grow, transport, and store microorganisms. To ensure thegrowth of microorganisms happens, it is effective if the medium contains all the nutrientsrequired.In natural habitats, microbes usually grow in a complex condition where it is mixed withmany kinds of species. This creates a problem for microbiologists because a single type ofmicroorganism cannot be studied adequately in a mixed culture. One needs a pure culture oraxenic culture, a population of cells arising from a single cell, to characterize an individualspecies. When isolating microbes from soils or aquatic habitats, microbiologists often beginby setting up conditions that favor growth of the desired microbes that is, they enrich for themicrobes of interest. This is followed by using other methods to obtain an axenic culturearising from a single cell.Pure culture technique is one of the methods that allows scientists to study a single speciesof a microorganism. It is derived from a mixed culture (contains a variety of species). Thistechniques is very significant for microbiologists to identify and study each species ofmicrobes. There are two basic methods for obtaining pure culture. They arestreak platemethodand thepour plate method.In microbiology,streakingis a technique proposed by Dr. Robert Koch which the mainidea is to isolate a pure strain from a single species of microorganism. Samples can be takenfrom mixed cultures and transferred to a new agar plate. This method establishes adilutiongradient. Dilution gradient means the concentration of the bacteria cells on the agar surfacebecomes lesserfrom the initial point of streaking to the last point of streaking. By using thismethod, we cannot guarantee the confluent growth of the bacteria cells on the last point ofstreaking where the bacteria cells deposited are less and not concentrated.Pour plate technique is a technique to introduce new bacteria culture into new media andallows it to distribute thoroughly.Most of the colonies grow within the medium and are smallin size and may be confluent.2.0 OBJECTIVESThe objectives of this experiment are:1.To understand the principles of pure culture techniques.

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Term
Summer
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Tags
Bacteria, Agar plate

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