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Set5Key - Bio 40 Problem Set 5 Key 1 You have identified...

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Bio 40 Problem Set 5 Key 1. You have identified three true breeding variants of E. coli phage T7. One variant causes large plaques, a second causes clear plaques, and a third causes blue plaques. (assume that wild type T7 plaques are small, cloudy, and colorless). A) You want to create a clear, large plaque forming phage. What strains do you need and what experimental steps must you undertake? 1. co-infect E. coli with the clear plaque mutant and the large plaque mutant. Use a high multiplicity of infection (MOI) to insure that individual cells get infected with both mutants. 2. harvest the resulting phage. 3. infect E. coli at a low MOI to insure that individual cells get infected with single phage. 4. screen for clear, large plaques. B) Now that you have a double mutant you coinfect it with the blue plaque variant, allow one round of growth, and plate the progeny phage for plaque morphology. You find: 1,027 large, clear 1,001 blue 425 large, blue 457 clear 112 clear, blue 101 large 37 large, clear, blue 35 wild type Draw the map. Show your distance calculations.
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