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enymes lab - Effects of pH and temperature on enzymes By...

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Absorbance Temperature of Water Bath ( ° C) Absorbance pH Effects of pH and temperature on enzymes By: Arjun Patel Partner: Sameep Chandrani 02/17/08 TA: Trotter Partner TA: Katherine
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Biology 102 – 26 Results: Table 1: Effects of Temperature on Extent of Browning Sample Temperature ( ° C) Absorbance after () min. Blank 0 1 0 0.081 2 Room Temperature 0.15 3 30 0.2275 4 40 0.206 5 60 0.1615 Graph 1: Effects of Temperature on Browning Rate After performing this part of the experiment and plotting the graph, we determined that the optimal temperature is 30 ° C. Table 2: Effects of pH on Extent of Browning Sample pH Absorbance after () min. Blank * 0 1 3 0.273 2 5 0.192 3 7 0.540 4 9 0.205
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5 11 0.158 Graph 2: Effects of pH on Extent of Browning After performing this part of the experiment and plotting the graph, we determined that the optimal pH is 7, neutral. Discussion: Enzymes are proteins that reduce activation for a reaction to occur. This means that less energy is required for a system to produce its products. Enzymes do not perform in all conditions though. There are ideal conditions in which enzymes operate. This does not mean that all enzymes have the same ideal conditions; every enzyme has different conditions in which it works in. In this lab, we tested the enzyme catecholase in two different types of conditions. We tested the enzyme in different pHs and temperatures
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