This preview shows page 1. Sign up to view the full content.
Unformatted text preview: Undifferentiated Early studies with hECS
showed hES grown on layer of mouse skin
cells remain undiffernitiated – did
not mature Research found Mouse skin cells produced
cytokines “Leukemia inhibitory factor” - LIF How Does LIF Work? LIF binds to 2 cell surface receptors LIF receptor gp130 receptor Activates STAT3 and Oct-4 1. Stem cell proliferation
2. Remain indifferentiated
Remain LIF New Methods to Produce ESCs
“Induced Pluripotent Stem
iPSs Stem Cell Breakthrough 2006
2006 Reprogrammed Stem Cells
Takahashi and Yamanaka - Kyoto University Normal adult cells “reprogrammed” into
becoming embryonic stem cells
becoming “Induced pluripotent stem cells” = “iPSs” iPS
What did they do?
1. Packaged 4 genes into a virus
virus Oct3/4 Sox2 C-Myc Kf4
1. Mouse skin cells (fibroblast) infected
2. Virus inserts 4 genes into the mouse
skin cells - “transduced”
skin Genes not found in these cells iPS
What were the results?
View Full Document
- Spring '11