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Unformatted text preview: ansduced” cells very similar to ESCs
1. ESC morphology and growth
2. Cells form all three germ layers
3. Cells injected into mouse embryo (blastocyst stage
developed with the embryo)
iPS Recent advances - July 2009 Two Chinese Scientist (Drs. Qi Zhou and
Shawrong Gao) produced iPC cell lines Injected iPCs cells into a dissected mouse embryo Embryos implanted into a female mouse (uterus) Produced live mice offspring Culturing iPS Cells Not culture on layer of
skin cells (mouse) Cultured on Matrigel Purified extracellular matrix iPS cells remain
immature Form colonies of iPS
cells Human iPS Cells How to change iPS cells into
mature cells? Initial colonies Embryoid bodies (EBs) Differentiate into mature cells Heart Brain Beta islet cells Potential of iPS Cells Autologous model Produce patient iPS Skin cells to iPS Culture produce
mature (or precursor
cells) Heart Neurons (dopamine) Pancreas (β islet) Stem Cell Genes
Studies with Adult Stem Cells
Adult CD34+ bone marrow stem cells CD34+
transplants Express 285 genes 106 potential transcriptional factors Regulate gene expression Stem cell genes identified by “gene array” Identifies active genes
Identifies Found genes that allow stem cells to stay stem
cells Termed “stemness genes”
Termed Why do stem cells express
these Hematopoiesis Cells mature and...
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This note was uploaded on 04/02/2013 for the course BIO 205 taught by Professor Dr.cady during the Spring '11 term at Bradley.
- Spring '11