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Resulting transduced cells very similar to escs 1 esc

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Unformatted text preview: ansduced” cells very similar to ESCs 1. ESC morphology and growth ESC 2. Cells form all three germ layers 2. 3. Cells injected into mouse embryo (blastocyst stage Cells developed with the embryo) developed iPS iPS Recent advances - July 2009 Two Chinese Scientist (Drs. Qi Zhou and Shawrong Gao) produced iPC cell lines Injected iPCs cells into a dissected mouse embryo Embryos implanted into a female mouse (uterus) Produced live mice offspring Culturing iPS Cells Not culture on layer of skin cells (mouse) Cultured on Matrigel Purified extracellular matrix iPS cells remain immature Form colonies of iPS cells Human iPS Cells How to change iPS cells into mature cells? Initial colonies Embryoid bodies (EBs) Differentiate into mature cells Heart Brain Beta islet cells Potential of iPS Cells Autologous model Produce patient iPS Skin cells to iPS Culture produce mature (or precursor cells) Heart Neurons (dopamine) Pancreas (β islet) Stem Cell Genes Stem Studies with Adult Stem Cells Studies Adult CD34+ bone marrow stem cells CD34+ transplants transplants Express 285 genes 106 potential transcriptional factors Regulate gene expression Stem cell genes identified by “gene array” Identifies active genes Identifies Found genes that allow stem cells to stay stem cells cells Termed “stemness genes” Termed Why do stem cells express these genes? these Hematopoiesis Cells mature and...
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