2CLabManual201303

2 fill both cuvettes with the solvent and wipe the

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Unformatted text preview: turns. Determining the Blank Cuvette 1. Obtain a pair of cuvettes. One will be used for the blank solution and the other will be used for the standard and unknown samples. 2. Fill both cuvettes with the solvent and wipe the outside with a Kimwipe to make sure it is clean and dry (no fingerprints). Be sure to always add enough solution to reach to within 1/8 of the triangular mark on the cuvette. This will insure that all incident radiation passes through the solution. It is also critical that the triangular mark on the cuvette be facing you when inserted in the sample holder for all measurements. 3. Insert one filled cuvette into the sample holder that is located under the hood to the left. Close the hood. 4. Calibrate to 0 absorbance by pressing the 0 ABS/100%T key. Remove the cuvette, insert the other cuvette, and read the absorbance for this second cuvette. If the reading is less than 0 absorbance, then this second cuvette will be used as the blank cuvette; if the reading is greater than 0 absorbance, then the first cuvette wil...
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