2CLabManual201303

3 obtain a pair of cuvettes one will be used for the

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Unformatted text preview: good results. Therefore, you will want to use a disposable pipet to put about 2 mL of it in a test tube and then dilute that with about 2 mL of the acidic solvent dispensed from the 50 mL buret. 3. Obtain a pair of cuvettes. One will be used for the “blank” solution and the other will be used for the standard and unknown samples. Determine which cuvette will be used for the “blank” solution by following the procedures given above under the section, “Determining the Blank Cuvette.” 4. Fill the cuvette selected for the blank to within 1/8 of the triangular mark on the cuvette with the acidic solvent dispensed from the 50 mL buret. 5. Rinse the other cuvette with a small portion of the diluted red solution (aquo product) and then fill it to within 1/8 of the triangular mark on the cuvette with this solution. 6. From the scanned spectra given in Figure 4 shown above, estimate the wavelength of the absorption maximum of the red colored complex. 7. Select a wavelength that is about 10 nm lower than the estimated absorption maximum determined in step 6. Calibrate...
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This note was uploaded on 04/09/2013 for the course CHE CHE 2C taught by Professor Nasiri during the Spring '07 term at UC Davis.

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