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Unformatted text preview: absorbance is simply the sum of the absorbances of the individual
species. When this is true, the individual concentrations can be determined from
spectrophotometric measurements. Because interactions often do arise, sometimes when
least expected, the absorption spectra of the species should be investigated when they are
separate and when they are simultaneously present to determine whether the absorbances
are indeed additive before any analytical spectrophotometric measurements. 50 Operation of GENESYSTM 20 Spectrophotometer
Spectrophotometers all contain some kind of light source (visible instruments often use
just a tungsten bulb); various mechanical and optical elements which are used to create a
directed and collimated beam; a wavelength selector of some sort (often either a
diffraction grating or a prism); an enclosed cell compartment to hold the absorbing
sample; a radiation detector (often a photocell or more commonly now a photodiode);
and some kind of readout device (e.g. a needle deflection on a meter).
It is important that you understan...
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This note was uploaded on 04/09/2013 for the course CHE CHE 2C taught by Professor Nasiri during the Spring '07 term at UC Davis.
- Spring '07