2CLabManual201303

In the first part of this experiment you will

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Unformatted text preview: be referred to as the chloro complex, while the red-colored mixture of the cis and trans isomers of the hydrolysis product, [Co(en)2(H2O)Cl]Cl2, will be simply referred to as the aquo complex. In the first part of this experiment you will determine the wavelength of maximum absorbance (λmax) for the two complexes. Then, you will prepare four solutions of each complex of known concentrations. You will then measure the absorbance of each solution using a GENESYSTM 20 spectrophotometer at the wavelength corresponding to the maximum absorbance for the chloro complex and will graph the data, with concentration on the x-axis and absorbance on the y-axis, to form a Beer's Law plot. By finding the slope and knowing the path length of your cuvette cell, you will be able to calculate the molar absorptivity coefficient at that wavelength for that complex. You will repeat this for the second wavelength that corresponds to the maximum absorbance position for the aquo complex. These measurements (i.e. at both...
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This note was uploaded on 04/09/2013 for the course CHE CHE 2C taught by Professor Nasiri during the Spring '07 term at UC Davis.

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