Unformatted text preview: the instrument following the procedure
given above under the section, “Calibrating the Instrument.” Once you have establish
0 absorbance using your blank solution, insert the sample cuvette and read the
absorbance. Record your measurement.
8. Remove the sample cuvette, increase the wavelength by two nanometers (nm), and
recalibrate the instrument using the blank cuvette. Re-insert the sample cuvette and
read the absorbance. Record your measurement.
9. Repeat step 8 until you have taken absorbance measurements every two nanometers
from 10 nm below your estimate to 10 nm above your estimate. This can be done
fairly quickly if you team up, with one person recalibrating, measuring, and calling
out the resulting absorbances and the other one recording the data. Switch roles half
way through the process so each partner has an opportunity to perform both tasks.
10. Empty the sample cuvette. Rinse it with deionized water, and carefully blot it dry
using a Kimwipe. 59 11. Repeat steps 2 and 5-10 above for the green solution...
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- Spring '07