2CLabManual201303

The light source in a genesystm 20 is an ordinary

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Unformatted text preview: d the operation of the GENESYSTM 20 before you begin this laboratory. The GENESYSTM 20 is an expensive and sensitive instrument and must be operated carefully and intelligently. The light source in a GENESYSTM 20 is an ordinary tungsten lamp whose radiation extends over the entire visible range. The light from the lamp passes through an entrance slit and is dispersed by a diffraction grating. The grating can be rotated so that a small band of selected wavelengths from the dispersed beam passes through an exit slit, and then through the cell (cuvette) containing the sample. The cuvettes used with a GENESYSTM 20 have a path length (internal diameter) of 1.00 cm. The light transmitted through the sample strikes a solid-state silicon detector that generates an electrical signal proportional to the radiant power (light intensity). The signal from the detector drives a meter that can be calibrated to read transmittance or absorbance. The calibration procedure entails setting 0 Absorbance at a given wavelength with a cuvette containing a reference or...
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