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Unformatted text preview: blank solution. The blank solution is missing the
component of interest, but is otherwise as identical as possible to the solution to be
analyzed for the component of interest. Typically, the blank solution is just the solvent.
This is required since the output of the lamp and the sensitivity of the detector varies with
wavelength. The electronics of the instrument automatically sets 100% absorbance. An
identical cuvette containing the solution of interest is then inserted into the spectrometer,
and the absorbance is read from a meter on the instrument. Both the calibration and the
reading must be done at the same wavelength. The reading for the solution then
represents the absorbance at the chosen wavelength due to the component of interest.
The calibration has accounted for any absorption (or reflection or scattering) of light by
the cuvette and other species in the reference solution.
GENESYSTM 20 Spectrophotometer
6. On / Off switch
Sample compartment hood
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This note was uploaded on 04/09/2013 for the course CHE CHE 2C taught by Professor Nasiri during the Spring '07 term at UC Davis.
- Spring '07