2CLabManual201303

You might try conditioning your index fingertip first

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Unformatted text preview: th your least-dexterous hand, use a rubber bulb fitted with an Eppendorf tip to draw the liquid a few centimeters above the mark on the pipet. If you keep the pipet bottomed, you can then remove the bulb and quickly seal the pipet mouth with the index finger of your "better" hand before the liquid level falls below the mark. You might try conditioning your index fingertip first by rubbing it gently in the palm of the other hand. If your finger is too wet, you can't create a small enough crack (see below), and if it is too dry, you can't get a good seal. Raise the over filled pipet vertically out of the vessel from which you are taking the measured sample and quickly put a beaker or some other waste receiver under it. Raise the mark on the pipet to your eye level, tilt the receiver slightly, and touch the pointed tip of the pipet to a dry spot on its sidewall. If you now slightly rock your index finger you can open and close a tiny crack at the mouth of the pipet and thereby allow the liquid level in the pipet to fall exactly to the mark on its shaft. (In this step some individuals have more success by slowly rotating the pipet using the thumb and t...
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