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least-dexterous hand, use a rubber bulb fitted with an Eppendorf tip to draw the
liquid a few centimeters above the mark on the pipet. If you keep the pipet
bottomed, you can then remove the bulb and quickly seal the pipet mouth with the
index finger of your "better" hand before the liquid level falls below the mark.
You might try conditioning your index fingertip first by rubbing it gently in the
palm of the other hand. If your finger is too wet, you can't create a small enough
crack (see below), and if it is too dry, you can't get a good seal.
Raise the over filled pipet vertically out of the vessel from which you are taking
the measured sample and quickly put a beaker or some other waste receiver under
it. Raise the mark on the pipet to your eye level, tilt the receiver slightly, and
touch the pointed tip of the pipet to a dry spot on its sidewall.
If you now slightly rock your index finger you can open and close a tiny crack at
the mouth of the pipet and thereby allow the liquid level in the pipet to fall exactly
to the mark on its shaft. (In this step some individuals have more success by
slowly rotating the pipet using the thumb and t...
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- Spring '07