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TC-2003-1 - Tissue Culture Exam I Name Answer questions...

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Tissue Culture Exam I Name____________________ Answer questions 1-40 on the computer-graded answer sheet. Answer questions 41 and 42 on this exam. 1. You order a culture of HeLa S-3. To subculture the cells you make four liters of Ham's F12 containing 10% fetal bovine serum. What volume of medium and what volume of serum will you add to make a total of 4 liters (4000 ml)? A. Add 3800 ml of medium and 200 ml of serum. B. Add 3600 ml of medium and 400 ml of serum. C. Add 3400 ml of medium and 600 ml of serum. D. Add 3200 ml of medium and 800 ml of serum.
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2. You also decide to freeze the HeLa cell line. Explain how to make 50 ml of freezing medium. How much DMSO would you add? How much Ham's F-12? A. Add 2.5 ml of DMSO to 47.5 ml of Ham's F-12. B. Add 5.0 ml of DMSO to 45 ml of F-12 C. Add 10 ml of DMSO to 40 ml of F-12. D. Add 45 ml of DMSO to 5 ml of F12. 3. Before freezing, you count your cell suspension in the presence of trypan blue. In the hemocytometer you see 286 greenish-white cells and 36 blue cells. What is the percentage viability of your culture? A. 11.2% C. 12.6% B. 88.8% D. 87.4% 4. The HeLa cell line is 5. What is the most frequent number of chromosomes found in the HeLa cell line? 6. What would be the best way of determining if the HeLa cell line can form a tumor (cancer)? 7. Does the HeLa cell line harbor retroviruses? A. Yes C. Maybe B. No D. Can't tell
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8. The Hela cell line would be expected to have G6PD, type ________. Thus, the HeLa S3 cell line ____________ have the expected type of G6PD. 9. Leonard Hayflick was reported to have said at the 1966 Tissue Culture meetings "I have just telephoned my wife who assured me that my worst fears are unfounded." Why did he say this? A. Because his wife worked with him in the laboratory and had just discovered that his daughter's cell line (WISH) was not really hers; there had been a mix up of amnion samples. B. Because his daughter's cell line had G6PD type A isoenzymes. His wife reassured him that she had discovered that an early sample of WISH cells had G6PD type B; thus the cell line had converted in culture from G6PD type B to A. C. He was joking. Something had happened to his daughter's cell line so that it appeared she had a parent who was black or of Mediterranean descent. D. Because his wife had repeated the isoenzyme test on the cells and found that the test had previously been done incorrectly. The cells really were G6PD, type B.
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