TC-2003-2

TC-2003-2 - Name_ Exam 2 Tissue Culture Questions 1-45 are...

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Name_______________________ Exam 2 Tissue Culture Questions 1-45 are worth 2 points and should be answered on the computer scan sheets. Questions 46-47 are worth 5 points and should be answered on this exam. The last 10 points comes from the article and critique. 1. There are two Mo cell lines. What is the difference between the original Mo cell line and the Mo-B cell line? A. There is no real difference. The ATCC called the first cells it received Mo-B when it received them and put this cell line into the cell collection. B. The Mo cell line is derived from spleen cells. The Mo-B cell line is derived from peripheral blood cells. C. The Mo cell line and the Mo-B cell line are both derived from the same source. When the lawsuit was filed, people who had the original Mo cell line changed the name to Mo-B so they would not be affected by the lawsuit. D. Genetics Institute changed the name of the original Mo cell line to Mo-B when they used the line to produce GMCSF. 2. The Mo-B cell line carries HTLV-II. What is HTLV-II and who discovered it? A. It is a human cancer virus discovered by Dr. Golde. B. It is a human cancer virus discovered by Dr. Hayflick. C. It is a HeLa virus discovered by Dr. Hayflick. D. It is a human cancer virus found by Dr. Gallo. 3. How did Genetics Institute use the Mo cell line to produce GMCSF? A. The GMCSF gene was isolated from the Mo cell line and put into the CHO cell line which produced GMCSF B. GMCSF was isolated from the cell culture medium in which the Mo cells were grown. C. The GMCSF gene was isolated from bacteria and put into the Mo cell line which produced GMCSF D. The GMCSF gene was isolated from a gene bank and put into the Mo cell line which then produced GMCSF.
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4. In the introduction to chapter 2 in the Animal Tissue Culture book, Gareth E. Jones states “Following the argument of the hypothesis for random X- chromosome inactivation proposed by Mary Lyon, as a gene dosage compensation mechanism in all placental mammals, it can be predicted that the dermis of female carriers of Duchenne muscular dystrophy will be populated by fibroblasts mosaic for the Duchenne genotype. By chance some cells will be expressing the gene products of the unaffected X chromosome, whereas others will have inactivated the normal X- chromosome and be expressing the Duchenne gene product.” (my underlining) 4. Why would the Lyon hypothesis for "random X-chromosome inactivation" be a gene dosage compensation mechanism"? A. If one of the two X chromosomes is inactive in female cells, then a female cell will not make twice as much of a gene product as a male cell which only has one X chromosome. B. If there is only one X chromosome in a male cell and two Xs in a female cell, then the one X chromosome in a male cell compensates by being twice as active as the X chromosomes in a female cell. C. Since X chromosomes are randomly inactivated in different cells in a
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This note was uploaded on 04/07/2008 for the course COS 314 taught by Professor Evans during the Winter '07 term at RIT.

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TC-2003-2 - Name_ Exam 2 Tissue Culture Questions 1-45 are...

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