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Unformatted text preview: iron. Microbiol. Field Observations unsuccessful don't tell us the whole study Key bacteria in sharpshooter gut have not been cultured 0.1mm Appearance of limited value Bacteriome dissected from anterior abdomen of H. vitripennis Orange-red portion- Baumannia only Yellow portion- Baumannia and Sulcia (Moran et al. 2003 Environmental Microbiology) 51 Thursday, December 6, 12 • Who Are They? 52 Thursday, December 6, 12 PCR and phylogenetic analysis of rRNA genes two obligate acteriomes bacteriomes DNA extraction PCR Makes lots of copies of the rRNA genes in sample PCR D Takiya la” (Gammaproteobacteria) oidetes) Moran et al. 2003 Environ. Microbiol. Moran et al. 2005 Appl. Environ. Microbiol. Phylogenetic tree rRNA1 Sequence alignment = Data matrix Yeast rRNA1 E. coli Humans A C A T A C AG T AGA C T AG rRNA1 5’ ...TACAGTATAGGT GGAGCTAGCGATC GATCGA... 3’ C E. coli omen of H. vitripennis C Yeast m A Sequence rRNA genes Humans A T AG T 53 Thursday, December 6, 12 5 Baumania is close relative of Buchnera symbionts of aphids Sharpshooters Aphids Aphids Aphids Ants Flies 54 Thursday, December 6, 12 • What Are They Doing? 55 Thursday, December 6, 12 56 Thursday, December 6, 12 Genome sequencing two obligate acteriomes bacteriomes DNA extraction D Takiya PCR Sequence the whole genome la” (Gammaproteobacteria) oidetes) Moran et al. 2003 Environ. Microbiol. Moran et al. 2005 Appl. Environ. Microbiol. m omen of H. vitripennis Predict functions by comparison to other organisms 57 Thursday, December 6, 12 5 Predict metabolic networks from Genome Wu et al. 2006 PLoS Biology 4: e188. 58 Thursday, December 6, 12 Baumannia is a Vitamin and Cofactor Producing Machine VITAMIN AND COFACTOR PRODUCING MACHINE Wu et al. 2006 PLoS Biology 4: e188. 59 Thursday, December 6, 12 Baumannia is a Vitamin and Cofactor Producing Ma...
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This document was uploaded on 04/13/2013.

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