2006 plos biology 4 e188 59 thursday december 6 12

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Unformatted text preview: chine NO PATHWAYS FOR ESSENTIAL AMINO ACID SYNTHESIS Wu et al. 2006 PLoS Biology 4: e188. 60 Thursday, December 6, 12 61 Thursday, December 6, 12 PCR and phylogenetic analysis of rRNA genes two obligate acteriomes bacteriomes DNA extraction PCR Makes lots of copies of the rRNA genes in sample PCR D Takiya la” (Gammaproteobacteria) oidetes) Moran et al. 2003 Environ. Microbiol. Moran et al. 2005 Appl. Environ. Microbiol. Phylogenetic tree rRNA1 Sequence alignment = Data matrix rRNA2 rRNA1 E. coli Humans A C A T A C AG T AGA C T AG rRNA1 5’ ...ACACACATAGGT GGAGCTAGCGATC GATCGA... 3’ C E. coli omen of H. vitripennis C rRNA2 m A Sequence rRNA genes Humans A T AG T rRNA2 5’ ...TACAGTATAGGT GGAGCTAGCGATC GATCGA... 3’ 62 Thursday, December 6, 12 5 63 Thursday, December 6, 12 Wu et al. 2006 PLoS Biology 4: e188. Thursday, December 6, 12 Genome sequencing two obligate acteriomes bacteriomes DNA extraction D Takiya PCR Sequence the whole genome la” (Gammaproteobacteria) oidetes) Moran et al. 2003 Environ. Microbiol. Moran et al. 2005 Appl. Environ. Microbiol. m omen of H. vitripennis Predict functions by comparison to other organisms 65 Thursday, December 6, 12 5 Sulcia makes essential amino acids 66 Thursday, December 6, 12 Sulcia makes essential amino acids ESSENTIAL AMINO ACID PRODUCING MACHINE 67 Thursday, December 6, 12 Sulcia makes essential amino acids Baumannia makes vitamins and cofactors Wu et al. 2006 PLoS Biology 4: e188. Thursday, December 6, 12 Symbiosis between Buchnera and aphids Organism Class of symbiosis A B Mutualism + + Commensalism + 0 Parasitism + - Symbiosis between bacteria & sharpshooters? 69 Thursday, December 6, 12 Symbiosis between Xylella and sharpshooters 2 symbionts in the sharpshooters: one make the vitamins and co-factors one make the essential amino acids sometime cross feed so that they can complete the process 3 players mutualism Organism Organism Class of symbiosis A B Mutualism + + Commensalism + 0 Parasitism + - Symbiosis between bacteria & sharpshooters? 70 Thursday, December 6, 12 Pierce’s Disease 71 Thursday, December 6, 12 Bacteria and archaea are key commensals of many eukaryotes 72 Thursday, December 6, 12 Don’t Neglect your Symbionts Thursday, December 6, 12...
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