study guide lab test 1

study guide lab test 1 - Lab 1 Iris diaphragm(lever adjusts...

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Lab 1 Iris diaphragm (lever)- adjusts light in-take below stage Objective lenses(4)- scanner(4X), low power(10X), high-dry(40X), and Oil Immersion(100X) Wet vs. dry mount – live vs. dead Light refraction- bending of light Index of refraction- a measure of the amount that light rays bend when passing from one medium to another Total magnification calculation- ocular X objective Resolution- the ability of an optical device to show two items as separate and discrete entities rather than a fuzzily overlapped image Clarity- clearness Depth of focus- the intensity at which the focus can see Working distance- space between microscope and specimen that organisms are viewable Immersion oil- substance used to avoid refraction at a glass-air interface when examining objects through a microscope Paracentered- Parfocal- for a microscope, remaining in approximate focus when minor focus adjustments are made Culture(wild vs. pure)- Aseptic technique- used to minimize contamination Aerobic – growth near the top Anaerobic – growth near the bottom Lab 2 Cultural characteristics (colonial characteristics) of bacteria, molds, and yeasts are studied by microbiologist for purposes of organism identification. See the characteristics on 38 Colony- a group of descendents of an original cell TNTC Lab 3 How and when to use a negative stain bacterial preparation – used when determining morphology when organisms are too delicate to heat fix Transfer needle (loop vs. stab) - loop used in grabbing media, stab when probing or examining media with a tool Staining (simple vs. differential) - only using one dye, several dyes and steps Negative stain technique – non-heat fixed to appear on a dark background Dorners nigrosin solution (nigrosin plus formalin) Acid dye vs. basic dye (nigrosin) - (methylene blue) – so that negatively charged organisms are repelled and are shown, where as basic dyes actually stain the organisms Coccus: cocci circle Bacillus: bacilli rod Spirillum: spirilla spiral
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Pairs-diplo chains-strepto (cocci and bacilli) clusters-staphylo 4’s-tetrad
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This test prep was uploaded on 04/07/2008 for the course BI 127 taught by Professor Unknown during the Fall '05 term at Grand Rapids Community College.

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study guide lab test 1 - Lab 1 Iris diaphragm(lever adjusts...

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