Unformatted text preview: a 5' phosphate DNA ligases catalyze nick sealing via 3 sequential reactions
enzyme becomes free the AMP increase the nucleophilicity for nucleophilic attack to seal the phosphodiester bond DNA polymerases synthesize DNA in a processive manner DNA polymerase accessory proteins Sliding clamp protein (-subunit) forms a ring around DNA association with polymerase then prevents polymerase from dissociating from DNA. Increases in processivity are achieved. 3D structure of a sliding clamp protein Sliding clamp protein
The sliding clamp protein (or the clamp) helps polymerase to bind to the template strand rapidly after each round of lagging strand synthesis
RNA pimer newly synthesized Lagging strand...
View Full Document