LS3.1 Week4 Lec11

Dna polymerases also possess exonuclease always

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Unformatted text preview: acts as a proofreading function 5'->3' exonuclease activity functions to remove RNA primers at the replication fork or damaged DNA during repair endonuclease doesn't need an end 3'-5' exonuclease 5' 3' 5'-3' exonuclease RNase H will cleave the red region 3' 5' RNA DNA 3' 5' DNA Polymerase Both prokaryotic and eukaryotic cells have multiple DNA Polymerases. DNA polymerase III is the major E. coli DNA replication enzyme. DNA polymerases are distinguished by their enzymatic activities, subunit composition and abundance has many activities 5' 3' polymerase activity for DNA synthesis 5' 3' exonuclease activity -- for removal of RNA primers 3' 5' exonuclease activity for repairing mistakes Removal of the RNA primer by RNase H and the 5' to 3' exonuclease activity of DNA polymerase 5'3' exonuclease and remove the last nucleotide phosphodiester bond between an adjacent 5' phosphate and 3'OH. DNA ligase creates a joining/ligating DNA ligase Uses the energy of ATP to form a phosphodiester bond between a 3' OH and...
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