Replication proceeds at both ends of the replication

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Unformatted text preview: proceeds at both ends of the replication fork Replication fork DNA polymerase Catalyze the synthesis of DNA Uses a single active site to catalyze the addition of any of the 4 dNTP's Specialized for either replication or repair In vitro replication assays revealed DNA synthesis could only occur on certain templates These structures do not serve as templates for a purified DNA polymerase. These structures do function as templates. All have free 3' end All have template-primer structure Primer:template junction - The template provides the ssDNA that directs the addition of each complementary deoxynucleotide - The primer, complementary to, but shorter than the template complementary base pairing Key components Reaction catalyzed by all DNA polymerases free 3' hydroxyl primer + dNTP primer-pN + pp primer extend one nucleotide Sugar-phosphate backbone: P-S-P-S-P Chemistry of DNA synthesis 2nd Component 2' deoxynucleoside triphosphates; dATP, dGTP, dCTP, dTTP c5 Which radiolabeled precursor will allow you to do a pulse chase experiment for DNA? 32P A dATP because that is what the...
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