LS3.1 Week4 Lec11

Double helix at the replication fork causes

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Unformatted text preview: ow the DNA strands to rotate about one another, relieving the supercoils Primase is a RNA polymerase Synthesizes short RNA primers (5-10 nucleotides long) to help the initiation of DNA replication Both leading and lagging strands require primase primase has to go on every time for the lagging strand due to the Okazaki fragment The role of the DNA helicase, SSB, and primase at the replication fork specifically cleave RNA and DNA hybrids RNase H specifically degrades RNA in the RNA:DNA Hybrid. RNase H removes all of the RNA primer except the ribonucleotide directly linked to the DNA end RNase H can only cleave bonds between two ribonucleotides The final ribonucleotide is removed by the activity of DNA polymerase how is it able to remove that one nucleotide How can DNA polymerases remove RNA primers? DNA polymerases also possess exonuclease always require activity and end 3'->5' exonuclease activity...
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This note was uploaded on 04/26/2013 for the course LIFE SCIEN 3 taught by Professor Wong during the Spring '13 term at UCLA.

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