Human Population Genetics LR

Human Population Genetics LR - Alu Fragment Screening Using...

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Alu Fragment Screening Using PCR David N Reynaud October 16, 2005 BSC2011L Section: 6773 Instructor: Sarah Haas
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Human Population Genetics Lab Report David N Reynaud; UFID: 7711-3183 BSC2011L Section:6773 Introduction In this experiment we examined the presence of a particular Alu element, which is a short repeating sequence of DNA distributed throughout primate genomes. Alu elements have recently incorporated themselves into the human genome and are a dimorphic insertion, which means that only some individuals will have the gene. The purpose of this lab is to screen a population of people for the presence or absence of one of these particular Alu elements, to determine whether or not this population is in Hardy- Weinberg Equilibrium. This equilibrium is one that probably exists only in theory, as it describes a population of organisms that does not undergo evolution. In order to be in Hardy-Weinberg Equilibrium, the population must meet with certain criteria. This theoretical population would be one that was very large, with no migration or gene flow between populations, have no mutations of genes, have random mating between individuals, and have no natural selection of certain genotypes over others. While this population is not one that exists in our study, the theory provides us with a standard set of values for our allele frequencies that we can compare to our test population in order to observe the process of evolution. Therefore the null hypothesis for this experiment would be that our population would be in Hardy-Weinberg Equilibrium. The alternate hypothesis would be that our test population would not be in Hardy-Weinberg Equilibrium, and would be considered an evolving population. Materials and Methods The Alu element that was examined in this experiment is called TPA-25, and is inserted within an intron of the tissue plasminogen activator gene. We are able to 2
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Human Population Genetics Lab Report David N Reynaud; UFID: 7711-3183 BSC2011L Section:6773 determine the genotype of an individual by examining the base pair length of the primers that flank the insertion site of the TPA-25 fragment. If there is a 400 base pair fragment,
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Human Population Genetics LR - Alu Fragment Screening Using...

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