Problemset3_F2012-1

You are asked to test the rate of the loss of color

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Unformatted text preview: rad research assistant and are given a preparation of purified enzyme X, a convenient chromogenic substrate which loses color as it is converted to product, and a compound your professor is convinced is an inhibitor of X’s activity. You are asked to test the rate of the loss of color at several different substrate concentrations keeping the concentration of enzyme constant, and to use the resulting measurements to describe the initial velocity and the Km for enzyme X and this substrate. With some help from the grad student who works on the next...
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