M2 W11

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Name
 
 
 
 1a.
 5
points.
A
stock
of
phage
T4
is
diluted
by
a
factor
of
10‐4
and
0.1
ml
of
it
is
mixed
with
 0.1
ml
of
108
E.
coli/ml.

The
mixture
is
plated
onto
an
agar
plate.

The
next
day
1130
plaques
are
 visible.

What
is
the
concentration
of
T4
phages
in
the
original
T4
stock.
 
 
 
 1.13
x
108
pfu/ml
 
 
 
 
 
 
 1b.
 8
points.
Temperature
sensitive
(ts)
mutations
of
phage
T4
grows
only
at
30°C
whereas
 the
wild
type
(ts+)
phage
T4
can
grow
at
both
30°C
and
40°C.

You
want
to
determine
the
 recombination
frequency
between
two
ts
mutations
(called
ts1
and
ts2).

An
E.
coli
strain
is
 coinfected
(at
high
m.o.i.)
at
30°C
with
a
wild
type
phage
stock
and
with
a
phage
that
contains
both
 ts
mutat...
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This note was uploaded on 05/05/2013 for the course LIFE SCIEN LS4 taught by Professor Jacobsen during the Fall '11 term at UCLA.

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