Caspie Final Project - Caspie-Final Report Chem 116-C /...

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Caspie-Final Report Chem 116-C / Section-0202 Craig Nutter April 19 th , 2007
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Abstract Throughout the Caspie lab experience, the hopes of our group was to formulate a means to extract the antioxidants present in various substances, how to measure their effectiveness, and to be able to use these factors in a useable application. Specifically to our group, oregano was used as the source of antioxidants. The means of extraction was through a substance prepared as an “oregano tea”. The oregano tea consists of a mixture of deionized water combined with ground oregano to form a solution that would be exposed to various stimuli. These stimuli were the independent variables throughout our individual structured labs. The independent variables were boiling for fifteen minutes, boiling for thirty minutes, burning, and cooling the oregano tea in an ice bath. Through experimentation, our group found that only burning the oregano had a significant impact on the effectiveness and/or amount of antioxidants present in the oregano tea water. This data was numerically expressed through the TEAC test which allowed our group to measure the change in absorbance after allowing the oregano tea water to react with the ABTS radical solution. These results mean that when placed into a solvent such as water, the ground oregano’s antioxidants were able to equally seep into the water with the same effectiveness despite heating or cooling the water. Only burning had an effect on the amount of antioxidants in the water which was a result of destroying or altering the effectiveness in their ability to react (or in its bodily function to prevent/fight off infection/disease) with the ABTS radical solution. Introduction: The applicability of the experiment and significance of antioxidants to the body shows just how vital of a role they play in the body. The ability of antioxidants to destroy potential harmful disease and sickness is reason enough for people to seek foods
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which contain high concentrations of them. The main threats to the body that antioxidants seek to destroy are known as reactive oxygen species. Antioxidants work by donating a hydrogen atom to the free radical contained in reactive oxygen species, which in essence terminates the potential harmful effects that can be experienced from the molecule. Experimentation specific to our group focuses on the high antioxidant concentrations contained in oregano. (The CASPiE Project) Oregano, normally used as a spice for heightening the taste of food actually provides many health benefits as shown throughout the experiment. (www.hort.purdue.edu) Oregano’s health benefits even contain more health benefits then fruits and vegetables gram for gram, oregano ranked above fruits and vegetables that are commonly thought to be high in antioxidants. Oregano contains 42 more times antioxidant activity than apples, 30 times more than
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This note was uploaded on 04/07/2008 for the course CHEM 116 taught by Professor Stevenson during the Spring '08 term at Purdue University-West Lafayette.

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Caspie Final Project - Caspie-Final Report Chem 116-C /...

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