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BSCI Translation II

BSCI Translation II - Kozak or Scanning model Internal...

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Ribosome nomenclature
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3-D Structure of Active Ribosome
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Prokaryotic mRNA
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Summary of Prokaryotic initiation
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Prokaryotic Initiation Factors Initiator tRNA with formyl-Met IF1, IF2, IF3
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Positioning the 30S at the start site
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Elongation
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Translation termination
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Prokaryotic vs Eukaryotic mRNA
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Prokaryotic vs Eukaryotic Ribosomes
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Eukaryotic initiation 1. Scanning or Kozak >90% of all eukaryotic mRNA 2. Internal ribosome entry site (IRES) 3. Re-initiation usually not allowed
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Translation initiation models
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Eukaryotic translation initiation eIF5 eIF1
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Eukaryotic AUG start site selection -consensus sequence driven -(A/G)CCATG G- Bioinformatics
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Unformatted text preview: Kozak or Scanning model Internal ribosome entry sites (IRES)-Picornovirus rapidly shut-down host protein synthesis 1. viral proteases cleave eIF-4G (polioviruses) 2. Viral-induced dephosphorylation of 4E-BP (EMCV)-cap independent translation initiation Viruses that use IRES Elongation factors EF-TU: *EF1α EF-Ts: *EFIβγ EF-G (prokaryotic): *EF2 *eukaryotic equivalent Eukaryotic Protein Termination Prokaryotic organisms 1. RF1 (UAA, UAG) 2. RF2 ((UAA, UGA) 3. RF3 (GTP-binding) Eukaryotic organisms 1. RF1 2. ??? Termination factors Assay for termination factors...
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