BSCI Translation III

BSCI Translation III - -phosphorylated eIF2 binds 100x more...

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Control of Eukaryotic Gene Expression
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Eukaryotic Translational Control Mechanisms Eukaryotic : -Secondary structure of mRNA -multiple AUG codons in 5’ UTR -protein binding sites in 5’UTR and 3’UTR -*phosphorylation of eIF-4E and eIF-4EBP -*phosphorylation of eIF2 alpha subunit -*viral proteases *:global inhibition
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Translation control via UTR binding proteins 5’UTR regulated genes : ferritin, mammalian sperm protamine, Drosophila sperm genes, riboswitches 3’UTR regulated genes : Large numbers of Drosophila development genes (nanos, bicoid, oscar, etc.
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Examples of UTR regulation elements
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Role eIF-2 in translation initiation eIF-2 Met-tRNA 40S eIF-2 eIF-2B Recyling eIF-2
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Regulation of ternary complex by phosphorylation of eIF2-alpha -phosphorylation of the SER51 on eIF2-alpha
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Unformatted text preview: -phosphorylated eIF2 binds 100x more tightly to eIF2b-prevents GDP to GTP exchange -blocks attachment of Met-tRNAi to 40S ribosome eIF-2 eIF-2B eIF-2 alpha kinase inactive Eukaryotic eIF-2 alpha kinases HRI : Heme deprivation PERK : ER stress caused by accumulation of unfolded proteins PKR: viral infection by double-stranded RNAs GCN2 : amino acid or glucose deprivation- phosphorylate Ser51 Conditions that induce eIF-2 alpha phosphorylation-Heat shock-Heme deprivation-Amino acid deprivation-Glucose deprivation-Viral infections-Disruption of Ca++-Differentiation-Unfolded protein response-Normal cell cycle: G2/M-Hibernation Control of Eukaryotic Gene Expression...
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This note was uploaded on 04/07/2008 for the course BSCI 110A taught by Professor Broadie/patton during the Spring '08 term at Vanderbilt.

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BSCI Translation III - -phosphorylated eIF2 binds 100x more...

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