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2 movies on binding of tata binding protein the

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Unformatted text preview: y on the frequency at which TFIID finds and binds the promoter. However, other factors can affect the ini1a1on of transcrip/on, even a]er the polymerase has bound. 5/23/11 TATA binding protein, a subunit of TFIID, has a structure like a saddle that fits perfectly onto the TATA box. It has dangling stirrups that will bind TFIIA and TFIIB. In a test tube, TBP can load RNA pol II onto a promoter all by itself. 2 movies on binding of TATA Binding Protein, the assembly of the eukaryotic transcription complex and the action of an enhancer http://www.youtube.com/watch?v=6tqPsI9aQA&feature=related http://www.youtube.com/watch?v=SMtWvD bfHLo&NR=1 Just as in E. coli, there are regulatory sequences that make up part of the eukaryo/c promoter. Again, as in E. coli, there regulatory sequences that might bind ac/vator proteins (which aaract TFIID, or ac/vate chroma/n) or repressor proteins, which block TFIID, or inac/vate chroma/n. “Enhancers” may be upstream or downstream of the gene, and can be very far away (20 kb?) so how can they ac/vate the gene? The promoter really aaracts just TFIID, right? 5/23/11 Enhancers tether ac/va/ng TFs to the general vicinity of the g...
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