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Unformatted text preview: t hormone-sensitive breast cancer promotes lung function by
supporting alveoli (in rodents but probably in humans) 5/23/11 Estrogens do this in part by stimulating transcription at genes
carrying a simple sequence called the Estradiol Response
Notice something funny about this sequence?
it’s a palindrome, kinda, almost- it looks the same whether you
read the top strand (5’ to 3’) or the bottom strand (5’ to 3’) Estrogen (EST) in bloodstream Cell membrane
Estrogen receptor protein
Estrogen binding causes dimerization
Dimerization causes nuclear
localization of the receptor and
binding to the Estrogen
Response Element 5/23/11 Binding of the receptor dimer to the ERE sequence stimulates transcription
by pol II Ah- now we know why this
is sequence symmetrical… EST …..GGTCAGCGTGACC….. ….600 bp later…Estrogen responsive gene …GGTCAGCGTGACC…
EST Demethylation of C and change of histone code at
Recruitment of activator proteins
Again, the SSD domain binds estradiol, the DBD
recognizes the enhancer (ERE) and the TAD does
whatever it does to induce transcription Complex interactions, but simple essential logic
estradiol Estradiol receptor Es...
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