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Ggtcagcgtgacc 600 bp laterestrogen responsive gene

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Unformatted text preview: t hormone-sensitive breast cancer promotes lung function by supporting alveoli (in rodents but probably in humans) 5/23/11 Estrogens do this in part by stimulating transcription at genes carrying a simple sequence called the Estradiol Response Element: GGTCAGCGTGACC Notice something funny about this sequence? …..GGTCAGCGTGACC….. …GGTCAGCGTGACC… it’s a palindrome, kinda, almost- it looks the same whether you read the top strand (5’ to 3’) or the bottom strand (5’ to 3’) Estrogen (EST) in bloodstream Cell membrane Estrogen receptor protein EST Nuclear membrane Estrogen binding causes dimerization EST EST Dimerization causes nuclear localization of the receptor and binding to the Estrogen Response Element 5/23/11 Binding of the receptor dimer to the ERE sequence stimulates transcription by pol II Ah- now we know why this is sequence symmetrical… EST …..GGTCAGCGTGACC….. ….600 bp later…Estrogen responsive gene …GGTCAGCGTGACC… EST Demethylation of C and change of histone code at ERE, Recruitment of activator proteins Again, the SSD domain binds estradiol, the DBD recognizes the enhancer (ERE) and the TAD does whatever it does to induce transcription Complex interactions, but simple essential logic estradiol Estradiol receptor Es...
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This note was uploaded on 05/17/2013 for the course BIS 100 taught by Professor Charles during the Winter '11 term at UC Davis.

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