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Link a bunch of amino acids together and you get a

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Unformatted text preview: uired enzyme (E): k1 kcat E + S <-> ES -> P + E Assuming Kcat >K1, v (rate) = k1 [E][S] (initially) and Vmax = kcat [E] at saturation Due to increase in [ES] Measures of enzyme q uality Vmax Km Temperature Affects Enzyme Ac<vity Gives a measure of turnover number of the catalyst (high is good) There’s an optimal temp and then the rate drops Gives a measure of how well substrate binds to enzyme (low is- sometimes- good) No catalyst Vmax i s the reaction rate when all enzyme is saturated Km is the substrate concentration needed to saturate half of enzyme and achieve 1/2 of V max It’s unfortunate that this is yet another K, but Km is a standard term What does a protein cost? (a lot) Individual amino acids are linked together via “ pep5de bonds” . Proteins vary in length from a dozen to a few thousand amino acids. Each protein has a precise series of amino acids that make up its primary structure. Link a bunch of amino acids together, and you get a long, flexible chain. It’s different with an enzyme. In real life, it costs 7 ATP (minimum) to add each amino acid to a protein. (not counting the cost of making the amino acid, or the cost of keeping up all the proteins us...
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