Homework 9 - LS5501 Biophysical Principles Dr Laity Fall...

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LS5501 Biophysical Principles Dr. Laity, Fall 2012 Homework #8 (6 December) Due Day Final Exam Return (20 points) 1. ( 2 points ) GFP is short for green fluorescent protein. GFP is used as an in vivo tag that can be attached to other proteins to monitor their cellular and sub-cellular localization. In addition to being good for you, β -carotene is a carotenoid that is actually used as an orange dye in foods like margarine. Given the above information, answer the following questions: a. Since it is quite large (26.9 kDa.), what problems might arise from using it as a tag? b. If applicable, what wavelength range would you expect to see GFP and β -carotene fluoresce, and absorb light? c. What is the difference between the origin of the orange color in β -carotene and the green color of GFP? 2. ( 2 points ) Problem 4 TSWP page 605 3. ( 3 Points ) Problem 8 TSWP page 605. 4. ( 3 points ) Problem 10 TSWP page 605.
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LS5501 Biophysical Principles Dr. Laity, Fall 2012 5. ( 1 points ) List two fundamental similarities between Raman and IR Spectroscopy and two differences as well.
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