mcb450-6s07 - Lecture 6 Lipids, Membranes, Transport Office...

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Lecture 6 Lipids, Membranes, Transport Office Hour Change: Today – None Tuesday, 2/6, 1:00-2:30 Wednesday, 2/7, 3:30-5:00 Thursday, 2/8, 1:00-2:30
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Ribonuclease Experiment Native ribonuclease is active as an enzyme. When placed in 8M urea (breaks H-bonds and β -mercaptoethanol (breaks S-S bonds) it denatures and is inactive. When 8M urea and β -mercaptoethanol are removed by dialysis, the enzyme re-folds into its native conformation and is active. Therefore primary structure dictates 3-D conformation.
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[email protected] http://folding.stanford.edu/
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Secondary Structure Prediction http://alexander.compbio.ucsf.edu/~nomi/nnpredict.html
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Fatty Acids No. C Atoms Name Melting Temp (C) 16 Palmitic acid (16:0)* 63.1 18 Stearic acid (18:0) 69.6 20 Arachidic acid (20:0) 76.5 16 Palmitoleic Acid (16:1) -0.5 18 Oleic acid (18:1) 13.4 * The first number in parentheses give the number of carbon atoms. The second gives the number of cis double bonds.
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Unsaturated Fatty Acids Naturally-occurring unsaturated fatty acids usually have a cis double bond between C 9 and C 10 , introducing a bend into the hydrocarbon tail. When more than one double bond exists in a polyunsaturated fatty acid, the double bonds are never conjugated. Rather there is an insulating methylene group.
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Some Fatty Acids C 16 satd C 18 satd C 18 (9-10 cis) C 18 9,12 cis C 18 9,12,15 cis C 20 6,9,12 cis
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trans Fatty Acids Are similar to saturated fatty acids in their physical properties, because they lack a kink in their tails. Are produced by partial hydrogenation of vegetable and fish oils. Raise plasma LDL cholesterol and triacylglycerol levels (bad) and lower plasma HDL cholesterol levels (also bad). Margarine has a high content of trans fatty acids.
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trans Fat in French Fries of Hospital Cafeterias Institution g trans fat in 6 oz. serving
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This note was uploaded on 04/07/2008 for the course MCB 450 taught by Professor Mintel during the Fall '07 term at University of Illinois at Urbana–Champaign.

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mcb450-6s07 - Lecture 6 Lipids, Membranes, Transport Office...

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