mcb450-10rs07 - Lecture 10 Enzymes: Kinetics, Specificity,...

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Lecture 10 Enzymes: Kinetics, Specificity, Regulation Office Hours Today (2//22/07) 1:00 – 2:00 PM Noyes 208
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Definition An enzyme is a biological catalyst. It accelerates the rate of a reaction, but does not affect free energy changes.
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Relationship Between Kinetics and Thermodynamics An enzyme lowers the free energy of activation for the reaction which is another way of saying it increases the velocity. It does not affect the overall free energy change.
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Some Properties of Enzymes Specificity Example: trypsin Regulation Coenzymes: Non-amino acid components necessary for the activity of some enzymes.
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A Multienzyme System
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Chemical Kinetics First-order reactions Rate is proportional to the concentration of [A].
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Chemical Kinetics Second-order reactions Rate is proportional to the product of the concentrations of [A] and [B].
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First Rate Study Adrian J. Brown, over 100 years ago. Studied the hydrolysis of sucrose in living cultures of yeast. Yeast cells secrete an enzyme into the medium that can cleave sucrose to glucose and fructose, which can enter the cell and be used as fuels. Made an unanticipated observation that the velocity of the reaction is not a linear function of sucrose concentration, but rather approached saturation.
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Model: E + S = ES -> P + E • v = k 2 E o S/(K M + S) = V max S/ (K M + S) – Where S = substrate concentration, E o = total enzyme concentration, K M is the Michaelis constant, and V max is the highest velocity
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This note was uploaded on 04/07/2008 for the course MCB 450 taught by Professor Mintel during the Fall '07 term at University of Illinois at Urbana–Champaign.

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mcb450-10rs07 - Lecture 10 Enzymes: Kinetics, Specificity,...

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