mcb450-16s07 - Lecture 16 The Citric Acid Cycle Office...

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Lecture 16 The Citric Acid Cycle Office Hours Today: 1:00-2:30 PM Noyes 208 Begin working on Problem Set 2 on the course website.
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Pyruvate Dehydrogenase Complex Pyruvate + NAD + + CoASH AcCoA + CO 2 + NADH + H + In the mitochondrion Large multienzyme complex. Can be seen in the electron microscope
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Pyruvate Dehydrogenase Steps i. Decarboxylation – Requires thiamine pyrophosphate, TPP. TPP typically is involved in the decarboxylation of α ketoacids.
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Thiamine Pyrophosphate, TPP Thiamine is membrane permeable. TPP, like most phosphorylated compounds, is impermeable.
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Pyruvate Dehydrogenase Steps i) Oxidation and acyl transfer – Requires lipoic acid Lipoic acid was first isolated and characterized by I. C. Gunsalus at UIUC. Lipoic acid in the reduced form has 2 SH groups. In the oxidized form there is a disulfide bond.
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Lipoic Acid Isolation Lipoic acid was initially isolated in crystalline form from a water-insoluble extract of beef liver. This isolation is one of the more spectacular achievements in the purification of natural products, since only 30 mg of crystalline lipoic acid was obtained from 10 tons of the liver residue. Mahler and Cordes, “Biological Chemistry”
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Lipoic Acid
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Pyruvate Dehydrogenase Steps i) Acyl transfer. The acetyl group is transferred from reduced lipoic acid to coenzyme A. Coenzyme A forms thioester bonds with
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mcb450-16s07 - Lecture 16 The Citric Acid Cycle Office...

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