Lab 2.xlsx - [S rate 10 15 20 25 30 45 60 1[S 0.6 0.75 0.9...

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[S] rate 1/[S] 1/rate 10 0.6 0.1 1.666667 15 0.75 0.066666666666667 1.333333 20 0.9 0.05 1.111111 25 1 0.04 1 30 1.1 0.033333333333333 0.909091 45 1.2 0.022222222222222 0.833333 60 1.25 0.016666666666667 0.8 [S] [V] inhibited enzyme rate 1 1/[S] 1/[V] 1/[inhibited] 5 0.2 0.1 0.2 5 10 10 0.35 0.17 0.1 2.857143 5.8823529412 15 0.5 0.22 0.066667 2 4.5454545455 20 0.55 0.27 0.05 1.818182 3.7037037037 25 0.59 0.3 0.04 1.694915 3.3333333333 30 0.63 0.35 0.033333 1.587302 2.8571428571 35 0.64 0.4 0.028571 1.5625 2.5 0.01 0.02 0.03 0.04 0.05 0.06 0.07 0.08 0.09 0.1 0.11 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 f(x) = 10.7890648669206 x + 0.586447399614811 R² = 0.99337024851865 Lineweaver--Burk Plot 1/[S] 1/[V] y - intercept: 0.5864 1 =0.5864 /???? Vmax = 1.705 (rounded to nearest thousandth) 𝐾? = /???? 10.789 Km = 18.449 (rounded to nearest thousandth) 8 10 12 f(x) = 42.8443916971641 x + 1.51487285316266 R² = 0.996997765996683 Lineweaver-Burk Plot (Inhibited Enzyme 1)
[S] [V] inhibited enzyme rate 2 1/[S] 1/[V] 1/[inhibited] 5 0.2 0.14 0.2 5 7.1428571429 10 0.35 0.18 0.1 2.857143 5.5555555556 15 0.5 0.22 0.066667 2 4.5454545455 20 0.55 0.25 0.05 1.818182 4 25 0.59 0.27 0.04 1.694915 3.7037037037 30 0.63 0.28 0.033333 1.587302 3.5714285714 35 0.64 0.3 0.028571 1.5625 3.3333333333 0.02 0.04 0.06 0.08 0.1 0.12 0.14 0.16 0.18 0.2 0.22 0 2

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