E55-2009 practice exam 1

E55-2009 practice exam 1 - E-55 Exam 1-2009 Name Page 1 of...

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E-55, Exam 1-2009: Name Page 1 of 9 BIOL E-55 Developmental Biology Exam 1 2009 A. Be sure to write your name on EACH PAGE of the examination. B. Read each question carefully and think before you write. If your answer cannot be read, it cannot be graded. C. If you do not show all of your reasoning, we cannot assign partial credit. D. The point value of each problem is noted. Allocate your time accordingly. IF YOU WRITE IN PENCIL YOU CANNOT ASK FOR A REGRADE Relax and do well!! Good luck! page 2______ (14 pts) page 3______ (14 pts) page 4______ (12 pts) page 5______ (12 pts) page 6______ (14 pts) page 7______ (12 pts) page 8______ (14 pts) page 9______ (8 pts) TOTAL /100 POINTS
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E-55, Exam 1-2009: Name Page 2 of 9 Multiple Choice (2 points each). Select the best answer from the choices provided. ______1. The function of cortical granules is to: A. alter the internal pH of the egg upon fertilization B. serve as storage site for Ca +2 ions C. attract sperm of the same species D. provide species compatibility match upon sperm binding E. prevent polyspermy ______2. Which of the following techniques provides information about the location of a protein in a tissue sample? A. RT-PCR B. in situ hybridization C. microarray D. immunohistochemistry E. gene targeting ______3. The discovery that ZP3 mRNA is present at high levels in the cumulus cells of the mammalian ovary could have been made by which of the following techniques: A. immunohistochemistry B. creation of a targeted mutation C. antisense knockdown D. in situ hybridization E. none of the above ______4. Fate mapping in a marine invertebrate finds that one blastula cell in the 32-cell stage blastula always gives rise to the cardiac myocardium (heart muscle). At the 32-cell stage, this labeled blastula cell is best described as: A. determined B. committed C. specified D. differentiated E. none of the above ______5. Spermatogenesis, unlike oogenesis, involves: A. cytoplasm conservation B. recycling of Golgi apparatus to form specialized secretory vesicles C. retention of mitochondria D. retention of tubulin proteins E. elimination of most cytoplasm during differentiation ______6. The notion of epigenesis encompassed the idea that A. embryos were pre-fashioned in all reproductive gametes B. embryos were pre-fashioned in certain genders’ gametes C. embryos were generated new from raw materials D. embryos were generated from religious convictions E. none of the above ______7. Resact, a protein found in sea urchin eggs, mediates A. fusion of the sperm to the jelly coat B. species recognition between the sperm membrane and the vitelline layer C. release of the acrosomal vesicle under the sperm membrane D. species attraction by serving as a chemotaxic agent for sperm E. binding of the acrosomal vesicle with the jelly coat
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E-55, Exam 1-2009: Name Page 3 of 9 ______8. Sea urchin eggs are placed into a salt solution that keeps their membrane potential positive. When sperm are added, the following will occur: A. fertilization with only 1 sperm, such that polyspermy is blocked B. fertilization will fail C. fertilization can occur, but polyspermy will be possible D. the majority of eggs will be subject to polyspermy E. the majority of eggs will prematurely enter mitosis
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