Lab 1 Page 1 Lab 1 Incubations (Page 2) Your Clan needs to place their Petri Plates in the appropriate SWA Boarding Groups on the Back Bench for Incubation (1 Clan = 4 Students). Clean-Up (Page 3) Lab Safety is Job One in MIC 101 and Lab Clean-Up is a critically important Component of Lab Safety. Lab 1 Exercises 1- Lighting your Bunsen Burner (Page 4) Prometheus stole Fire from Zeus for you, so put it to good Use. 2- Sterilizing your Inoculating Loop (Page 5) Basic. Fundamental. Easy. And Important. 3- Streaking (Pages 6-9) I like Peanut M&Ms. But I like the Red ones best so I eat them last. I always take a Package of Peanut M&Ms, pour it out onto a Table, and then spread them out with my Hand so they’re just one Layer Thick, and then use my Finger to move-out the Brown ones, then the Yellow ones, then the Green ones and finally the Red Ones. We’ll be doing roughly the same Thing Today when we streak Bacteria onto a Nutrient Agar Plate. We’ll transfer the Bacteria from an isolated Colony -- a small Button of Bacteria -- on one Plate to another Plate. Then we’ll spread them out so we have individual Bacteria separated out on this new Plate. Each of these Bacteria will divide and divide and divide and produce a new Colony. 4- Isolations (Pages 10 and 11) In Today’s Lab you’ll start a Quarter-Long Project to isolate and obtain Pure Cultures of Bacteria from Soil Samples, and from “Patient” Samples. Rogues Gallery (Pages 12-18) We’re going to be working extensively with seven (7) different Bacteria. We’ve invited these eight to Lab and have placed them on the Back Bench so you can get an Idea of their Colony Morphology and see some of the different Media we’ll be using during the Course. Background for Lab 1 Exercises (Pages 19-33) Here be Exam Questions. Or more precisely, the Answers to Exam Questions.

Lab 1 Page 2 Lab 1 Incubation Boarding Groups (per Clan [2 Trans-Lab Partners]) 04°C (Cold Room/Refrigerator) Dark Blue Tape, Farthest to the Left 1 Patient Skin Swab Plate 1 Patient Rectal Swab Plate ___________________________________________________________ 25°C (Room Temperature [American]/Cool) Cabinet under the Bench by the Windows 4 Pseudomonas sp. Soil Isolate Sodium Benzoate Tubes ___________________________________________________________ 30°C 24 H (Outside Temperature/Warm) Light Blue Tape No Plates ___________________________________________________________ 30°C 48 H (Outside Temperature/Warm) Light Blue Tape 4 Bacillus sp. Soil Isolate Plates (Nutrient Agar/Black Stripe) ___________________________________________________________ 37°C 24 H (Body Temperature/Blood Heat [British]) Orange Tape 4 Patient Rectal Swab Isolate Plates (Nutrient Agar/Black Stripe) 4 Patient Rectal Swab Isolate Plates (EMB Agar) ___________________________________________________________ 37°C 48 H (Body Temperature/Blood Heat [British]) Orange Tape, Farthest to the Right 4 Patient Skin Swab Isolate Plates (YGC Agar/Red Stripe)

Lab 1 Page 3 Lab 1 Clean-Up The Clean-Up at the End of each Lab Session is so Important that it gets its own Page at the Beginning of each Lab. You’ll be working with and contaminating a lot of Glassware, Pipettes, Slides, and Petri Plates. It is extremely Important that all used Glassware, Pipettes, Slides, and Petri Plates be placed in the appropriate Waste Container. When in doubt, Ask!