MICROBIOLOGY2SUB-STAGESREACTANTSPRODUCTSATP CONTRIBUTIONLOCATIONGlycolysisGlucose(1molecule)Pyruvic acid(2 molecules)Net gain of 2 ATPmolecules via substratelevel phosphorylation.Cytoplasm ofcellsLink Process(pyruvic acidto acetyl-CoA)Pyruvic acid(2molecule)Acetyl-CoA(2 molecules)No direct ATP isformedMitochondrialmatrixKrebs cycleAcetyl-CoA+oxaloaceticacid(OAA)Differentintermediatecarboxylicacidcompoundslike succinicacid, malicacid,etc.2 ATP formed viasubstrate levelphosphorylationMitochondrialmatrixElectrontransportsystem (ETS)NADH+H+,FADH 2metabolicwater alongwith ATP viaoxidativephosphorylationTotal of 34 ATP formed(6 ATP from glycolysis,6 ATP from linkreaction and 22 ATPfrom Krebs cycle)InnermitochondrialmembraneGlycolysis is a system of reactions that take glucose and turn it into energy, and does notrequire oxygen. It splits two carbon molecules called pyruvates. Nearly every living organismperforms glycolysis, making it an ancient metabolic pathway. Glycolysis takes place in thecytosol of a cell, and it can be broken down into two main phases: the energy requiring phase
