Chapter 17

At a throughput rate of 2lh average residence me is 5h

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Unformatted text preview: E. coli NM989 is an excision defec/ve λ lysogen where the T4 DNA ligase gene is transcrip/onally controled by the pL promoter and the cI repressor. •  Growth phase is 30oC and induc/on phase is 42oC. Growth phase working vol. = 10L and induc/on phase working vol. = 5L. •  Cells doubled every 63 min in growth phase and every 77 min in induc/on phase. At a throughput rate of 2L/h, average residence /me is ~5h in growth phase and ~2h in induc/on phase. •...
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This note was uploaded on 06/09/2013 for the course BIOL 432 taught by Professor Glick during the Winter '13 term at Waterloo.

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