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Chapter 17

Dicult to maintain batch is the industry standard

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Unformatted text preview: lls in a more uniform physiological state •  Con/nuous runs may be so long that recombinant cells lose their plasmid, and sterility is difficult to maintain •  Batch is the industry standard •  Regardless of the bioreactor and whether the run is batch, fed ­batch or con/nuous, it is desirable to monitor and control pH, temperature, dissolved O2, s/rring rate and foaming. See pages 687 ­692 High Density Cell Cultures •  Generally, the greater the final cell density the greater the amount of product •  In some cases, cell...
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