Chapter 17

E fedbatch culture is the most eecve way to aain a

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Unformatted text preview: densi/es of >50 g DW/L can be obtained when using fed ­batch cultures (where DW = 20 to 25% WW) •  Past a certain point, increasing the nutrients in the growth medium can be inhibitory to cell growth •  Adding media components a li`le bit at a /me (i.e. fed ­batch culture) is the most effec/ve way to a`ain a high cell density •  At high cell densi/es, oxygen quickly becomes limi/ng See page 693 Large Scale Produc/on of T4 DNA Ligase •...
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This note was uploaded on 06/09/2013 for the course BIOL 432 taught by Professor Glick during the Winter '13 term at Waterloo.

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