2Alecture+8

In real life it costs 7 atp minimum to add each amino

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Unformatted text preview: ly substrate, not product. 8 1/24/10 When [S] >> [E], the velocity simply reflects the catalytic rate of the enzyme- increasing substrate concentration doesn’t increase velocity, because the substrate’s stuck waiting for an available enzyme… Why does the catalyzed reac>on rate fla_en out at high [S]? •  The back reac>on starts up •  The reac>on can only go so fast ­ that’s the top speed •  The enzyme is too busy to cope with addi>onal substrate kf Reaction without enzyme: S (substrate) -> P (product) With a required enzyme (E): k1 Measures of enzyme quality kcat E + S <-> ES -> P + E Vmax v (rate) = k1 [E][S] (initially) and Vmax = kcat [E] at saturation Km Due to increase in [ES] Gives a measure of turnover number of the catalyst (high is good) Gives a measure of how well substrate binds to enzyme (low is good) Vmax is the reaction rate when all enzyme is saturated Km is the substrate concentration needed to saturate half of enzyme and achieve 1/2 of Vmax Temperature Affects Enzyme Ac<vity There’s an optimal temp and then the rate drops What does a protein co...
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This note was uploaded on 06/13/2013 for the course BIS 2A taught by Professor Grossberg during the Winter '08 term at UC Davis.

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