Probelm set 2-UNEX Spring 2013

5pts b show the insertion point and orientation of

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Unformatted text preview: n. (3pts) c) In the use of each of these Hfr strains, state which allele you would select to obtain the highest proportion of Hfr exconjugants. (3pts) Q4. An ade+ arg+ cys+ his+ lys+ pro+ bacterial strain is known to be lysogenic for a newly discovered phage, but the site of the prophage is not known. The bacterial map is cys lys arg his pro ade The lysogenic strain is used as a source of the phage, and the phages are added to a bacterial strain of genotype ade-­‐ arg-­‐ cys-­‐ his-­‐ lys-­‐ pro-­‐. After a short incubation, samples of these bacteria are plated on six different media, with the supplementation indicated in the following table. The table also shows whether colonies were observed on the various media. Nutrient supplementation in medium -­‐-­‐-­‐-­‐-­‐-­‐-­‐-­‐-­‐-­...
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This note was uploaded on 06/16/2013 for the course BIO 210 taught by Professor Sarni during the Summer '11 term at UCLA.

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