lecture 17-2008-stemcells

lecture 17-2008-stemcells - 2/22/08 Genetics Applications...

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2/22/08 Genetics Applications II: Cloning/ stem cell research (Ch. 9: 143-144) Lecture outline Genetic applications Cloning Stem cell research Cloning - what is it? How does it work? Whole organism cloning steps: 1. eggs coaxed to mature in culture dish 2. needle used to remove egg “plug” 3. egg genetic material removed (nucleus) 4. genetic material from other cells picked up 5. other genetic material (e.g., Dolly’s DNA) inserted into egg a. called “somatic cell nuclear transfer” 6. injected egg exposed to chemicals/ growth factors/ shock 7. activated egg divides 8. hollow ball of cells (blastocyst) forms after a few days 9. implant cells in uterus of surrogate mother Stem cell research – a simple variation Stem cells (or “totipotent” cells) Have ability to form into different cell types The process: Do steps 1-8: Remove DNA from egg Insert genetic material of individual you are cloning Expose eggs to growth factors Grow egg to blastocyst (cell ball stage)
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This note was uploaded on 04/07/2008 for the course BIOL 102 taught by Professor Storfer during the Spring '08 term at Washington State University .

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lecture 17-2008-stemcells - 2/22/08 Genetics Applications...

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