GENETICLAB - #9-#11Transformation of E.Coliwith the plasmid...

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Unformatted text preview: #9-#11Transformation of E.Coliwith the plasmid pAMPPreparation of plasmid DNA (mini-prep)Restriction analysis of mini-prep DNAINTRODUCTIONWithin the first section of the laboratory experimentation, E.Colicells are transformed with DNA plasmids in order to obtain ampicillin resistance genes. Transformation occurs when different genetic traits are incorporated into a cells DNA through absorption. Transformation is often induced within experiments and it is used commonly within cloning and expression in laboratory experimentation. After this transformation, the E.Coli will be placed upon agar plates containing ampicillin. This procedure will test if transformation has occured. The E.Coli that have not absorbed the ampicillin resistant genes will not grow on the culture. The E.Colithat have absorbed the resistant genes will continue to grow on the culture despite the prescence of ampicillin in the agar.Within the second section of the laboratory experimentation, a "mini-prep" procedure is used to identify whether ampicillin resistance in the E.Coliwas caused by the transformation of pAMP into the genetic coding. The "mini-prep" is commonly used to monitor traits on a molecular level.Within the last section of the laboratory experimentation, these transformed cells are placed on an agarose gel with a control group of cell with pAMP. Therefore, throughout this gel electrophoresis one can see if the transformed...
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GENETICLAB - #9-#11Transformation of E.Coliwith the plasmid...

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