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Unformatted text preview: hormone and there is also protein-bound hormone
-There is an equilibrium bet ween hormones bound to a carrier and free hormones What determines the amount of active
hormone in the plasma?
H H H
(depends on free hormone and
number of receptors)
Target cell H-carrier
H H Hormone + Protein <--> Hormone Bound to Protein
-If you take away hormone, you're going to shift -----> this way
-Similarly, if you take away the hormone bound protein, you will shift <------- that way
-There are really small concentrations of hormones in the body (nanomolar) to produce a
-Metabolism and production will also play on this eqm (if you produce more hormone, it will shift the
e quation --> How are hormones detected/measured?
Hormones are exceptionally potent chemicals
Nanomolar (10-9 M) to picomolar (10-12 M) (Range) Hormone measurements (sensitive method):
Immunoassay: tagged antibody specific to hormone
(detection in blood and urine)
Immunohistochemistry: detection in tissue
-Advantage: you know where the hormone is in relation to that cell. Tells us where the hormone is in the body!
-Rarely used in medicine. We use it to se BRCA2 hormones (cause breast cancer in women) Both use antibodies that specifically bind to part of the hormone Case Study: Man with hyperglycemia
A 51-year-old man visits his doctor complaining of decreased alertness,
withdrawal, irritability and sleepiness. He is not obese.
Fasting blood glucose concentration = 9 mmol/l
(normal range = 4 - 6 mmol)
Plasma insulin concentration = 80 IU/ml
(normal range = 5 - 25 µU/mL)
1) What are the possible causes of this disease?
2) How would you treat him given each possible cause?
Study Next class
Hormone receptors and signal transduction...
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- Summer '12