Population Genetics (1).docx - Population Genetics Before...

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Population Genetics Before beginning this lab, be sure to review the lab introductions. Note : In the following experiments on gene pool, gene frequency, and genetic diversity, assume there are four alleles for color and that they are all homologous. Experiment 1: Genetic Variation Genetic variation is simply the genetic difference within or between populations observed either in the gene pool or in the gene frequency. Consider the following two populations of butterflies (Figure 1): Figure 1: Butterfly Populations Assumptions : Both populations in Figure 1 contain the same four colors (yellow, orange, white, and orange/white combo) of butterflies, thus the gene pool is the same. However, the distribution of colors within that population is different. As a result, their gene frequencies are different, resulting in genetic variation between the two populations. In this experiment, you will create two different populations and investigate their genetic variation. 1
Materials Blue Beads Red Beads Green Beads Yellow Beads (2) 100 mL Beakers (2) 250 mL Beakers Permanent Marker Procedure Part 1: 1. Pour and mix 50 blue beads and 50 red beads into a 250 ml beaker. Stir to mix the beads. Without looking, select 50 beads from the 250 mL beaker and place them in a 100 mL beaker. Label this beaker as Beaker #1. 2. Pour and mix 50 green beads and 50 yellow beads into a second 250 mL beaker. Stir to mix the beads. Without looking, take 20 beads from the 250 mL beaker and place them in the other 100 mL beaker. Label this beaker as Beaker #2. 3. Take a picture of both beaker 1 and 2. Include a tag with your name and upload this picture into Moodle where requested. 4. Answer the post lab questions for Part 1 below. Procedure Part 2: 1. Dump the contents of Beaker #2 into Beaker #1 and mix the contents thoroughly. Pour approximately half the beads from Beaker #1 back into Beaker #2. 2. Answer the post lab questions for Part 2 below. 3. Note: when done, keep the beads in their beakers for use in the next exercise. Post-Lab Questions Part 1 1. What is the initial gene pool of beaker #1? (i.e., what colors are available in this beaker?) 2. What is the initial gene pool of beaker #2? (i.e., what colors are available in this beaker?) 3. What is the initial gene frequency of beaker #1? ( Consider the total number of beads and what each color contributes to that total.) 2
4. What is the initial gene frequency of beaker #2? ( Consider the total number of beads and what each color contributes to that total.) 5. What can you say about the genetic variation between these populations (the “population” in beaker 1 versus the “population” in beaker 2)? Part 2 6. What is the new gene pool of beaker #1? 7. What is the new gene pool of beaker #2? 8. What is the new gene frequency of beaker #1? 9. What is the new gene frequency of beaker #2?

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